Journal: Cell reports

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

doi: 10.1016/j.celrep.2025.115374

Figure Lengend Snippet: (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

Techniques: Immunostaining

Journal: Cell reports

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

doi: 10.1016/j.celrep.2025.115374

Figure Lengend Snippet: (A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .

Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

Techniques: Membrane, Marker, Immunostaining

Journal: Cell reports

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

doi: 10.1016/j.celrep.2025.115374

Figure Lengend Snippet: (A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .

Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

Techniques: Immunostaining

Journal: Cell reports

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

doi: 10.1016/j.celrep.2025.115374

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

Techniques: Recombinant, Modification, Saline, RNAscope, Multiplex Assay, Electron Microscopy, Negative Control, Software, Cell Culture, Fluorescence, Microscopy, Hybridization

Journal: iScience

Article Title: Disuse plasticity limits spinal cord injury recovery

doi: 10.1016/j.isci.2025.112180

Figure Lengend Snippet:

Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy

Journal: iScience

Article Title: Disuse plasticity limits spinal cord injury recovery

doi: 10.1016/j.isci.2025.112180

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy